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Test Code LAB919 Human Immunodeficiency Virus 1 RNA Quantitative PCR

Additional Codes:

HIV1RNA

Collection:

Specimen: Blood

Container: One White EDTA PPT Tube (preferred) . One 4 ml Dark Lavender ( 3 ml minimum) is also acceptable for pediatric collection or PPT unavailability. If a Dark Lavender tube is collected,  a separate tube is required if multiple tests are ordered.

Volume: 6 mL; Minimum 3 mL

Unacceptable:  Heparinized specimens or specimens exposed to repeated freeze/thaw cycles.

Storage Instructions:

For PPT, spin and refrigerate.  For Lavender, centrifuge within 6 hours of collection, aliquot & freeze 2.0 ml (minimum 1.2ml) of EDTA plasma.  Ship specimen by overnight delivery, frozen on dry ice.

Turnaround Time (TAT):

Results reported on day testing is performed.

Test Availability:

Performed twice a week - day varies. Observed holidays may adjust testing schedule.

Assay:

1.1 ml plasma

Request Form:

Routine Laboratory Request Form

Send To:

UMC MAIN LABORATORY Specimen Receiving – EH319 Children’s Hospital

Reference Interval:

Not Detected 

Interpretive Data:

The HIV-1 RNA assay methodology is polymerase chain reaction (PCR). The PCR primers and partially double-stranded DNA probe targets two regions of the HIV-1 gene: the integrase region of the polymerase gene of HIV and the long terminal repeat (LTR) promoter. The linear range of this assay is between 10 and 10,000,000 copies/mL. 

 

Performed in

UMC - Molecular Pathology