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Test Code LAB8883 St. Louis Encephalitis Antibody Panel, IgG and IgM, Spinal Fluid

Additional Codes

STLPC

Useful For

Aiding the diagnosis of St. Louis encephalitis using spinal fluid specimens

Method Name

Immunofluorescence Assay (IFA)

Specimen Type

CSF


Ordering Guidance


This assay detects only St. Louis virus. For a complete arbovirus panel, order ABOPC / Arbovirus Antibody Panel, IgG and IgM, Spinal Fluid.

 

New York State Clients: This test is not available for specimens originating in New York.



Specimen Required


Container/Tube: Sterile vial

Preferred: Vial number 1

Acceptable: Any vial

Specimen Volume: 0.8 mL


Specimen Minimum Volume

0.7 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 14 days
  Frozen  14 days
Medical University of South Carolina Note:

On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318 
 
Off-campus collections: 
Do Not Spin down
Specimen to be stored/transported at Refrigerated temp. 

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Reference Values

IgG: <1:1

IgM: <1:1

Reference values apply to all ages.

Interpretation

Detection of organism-specific antibodies in the cerebrospinal fluid (CSF) may suggest central nervous system (CNS) infection. However, these results are unable to distinguish between intrathecal antibodies and serum antibodies introduced into the CSF at the time of lumbar puncture or from a breakdown in the blood-brain barrier. The results should be interpreted with other laboratory and clinical data prior to a diagnosis of CNS infection.

Method Description

The indirect immunofluorescent antibody (IFA) assay is a 2-stage “sandwich” procedure. In the first stage, the patient cerebrospinal fluid (CSF) is diluted in Pretreatment Diluent for IgM and phosphate buffered saline (PBS) for IgG, added to appropriate slide wells in contact with the substrate, and incubated. Following incubation, the slide is washed in PBS which removes unbound CSF antibodies. In the second stage, each antigen well is overlaid with fluorescein-labeled antibody to IgM and IgG. The slide is incubated allowing antigen-antibody complexes to react with the fluorescein-labeled anti-IgM and anti-IgG. After the slide is washed, dried, and mounted, it is examined using fluorescence microscopy. Positive reactions appear as cells exhibiting bright apple-green cytoplasmic fluorescence against a background of red negative control cells. Semi-quantitative endpoint titers are obtained by testing serial dilutions of positive specimens.(Package inserts: Arbovirus IFA IgM and Arbovirus IFA IgG Instructions for Use. Focus Diagnostics; Rev 02, 05/01/2018)

Day(s) Performed

May through October: Monday through Friday

November through April: Monday, Wednesday, Friday

Report Available

Same day/1 day to 4 days

Specimen Retention Time

2 weeks

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86653 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
STLPC St. Louis Enceph Ab Panel, CSF 96254-8

 

Result ID Test Result Name Result LOINC Value
26367 St. Louis Enceph Ab, IgG, CSF 21509-5
26368 St. Louis Enceph Ab, IgM, CSF 21510-3

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.

Testing Algorithm

For more information see Mosquito-borne Disease Laboratory Testing .