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HelpTest Code LAB8817 D-Lactate, Plasma
Additional Codes
DLAC
Useful For
As an adjunct to urine D-lactate (preferred) for the diagnosis of D-lactate acidosis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Plasma NaFl-KOxOrdering Guidance
Urine is the preferred specimen for D-lactate determination, order DLAU / D-Lactate, Urine.
For determination of L-lactate (lactic acid), order LACS1 / Lactate, Plasma
Specimen Required
Supplies: Sodium Fluoride/Potassium Oxalate Tube, 2 mL (T275)
Collection Container/Tube:
Preferred: Sodium fluoride/potassium oxalate tube
Acceptable: Green top (sodium heparin)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge, aliquot plasma into a plastic vial, and freeze immediately.
NOTE: If collecting in sodium heparin tubes, centrifugation must occur within one hour of collection.
Specimen Minimum Volume
0.15 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma NaFl-KOx | Frozen (preferred) | 91 days | |
| Ambient | 91 days | ||
| Refrigerated | 91 days |
On-campus collections: Aliquot plasma in plastic vial, and Freeze immediately
Off-campus collections: Centrifuge and aliquot within 30 mins. Specimen to be stored/transported at Frozen temp.
Reference Values
0.0-0.25 mmol/L
Day(s) Performed
Monday, Thursday
CPT Code Information
83605
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DLAC | D-Lactate, P | 14045-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8878 | D-Lactate, P | 14045-9 |
Interpretation
Increased levels are consistent with D-lactic acidosis. However, because D-lactate is readily excreted, urine determinations are preferred.
Method Description
Plasma is spiked with a mixture of internal standards and evaporated. The dry residue is derivatized with DATAN to form the diastereomeric molecules, then acidified and extracted with ethyl acetate. After evaporation, the dry residue is again derivatized to form trimethylsilyl (TMS) esters at the carboxylic acid moiety. Specimens are then analyzed by capillary gas chromatography mass spectrometry selected ion monitoring using positive chemical ionization, with chromatographic separation of the L,L- and D,L-diastereoisomers of derivatized lactate, corresponding to L-Lactate and D-Lactate, respectively.(Ding X, Lin S, Weng H, Liang J. Separation and determination of the enantiomers of lactic acid and 2-hydroxyglutaric acid by chiral derivatization combined with gas chromatography and mass spectrometry. J Sep Sci. 2018;41(12):2576–2584)
Report Available
3 to 6 daysSpecimen Retention Time
1 monthReject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Gas Chromatography Mass Spectrometry (GCMS) Stable Isotope Dilution Analysis