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Test Code LAB2225 Heparin Associated Anti-Platelet IgG Antibody with Reflex to Serotonin Release Assay (SRA)

Important Note

Specimens must be received in labortatory prior to 9AM to be resulted that day.

Samples from OUTSIDE of MUSC will not be accepted due to high volume of tests from MUSC patients.

Additional Codes:

PF4/Reflex

Includes:

Heparin Associated Anti-Platelet Antibody (PF4 antibody) with reflex to Serotonin Release Assay (SRA). If the PF4 antibody is positive, SRA is performed at an additional charge.

For orders from MUSC and Non-MUSC providers, the SRA will automatically be sent to a reference lab when the PF4 Antibody is positive (>0.500 OD). Specimens received Monday – Friday will be sent out same day. Specimens received on Saturday and Sunday will be sent out on Monday. 
 

Notes:

Do not draw from a heparinized line. Samples anticoagulated with heparin are not suitable for testing with this assay.

Include Heparin type and date/time of last dose.

Hemolyzed specimens are unacceptable.

Collection:

Specimen: Blood

Container: Two 4 ml Serum Separators or one 7 ml Plain Red Top Tube

Volume: 7 mL; Minimum 5 mL

 

Rejection Criteria: Gross helolysis, lipemia, icteric serum.

Storage Instructions:

Serum should be separated from red cells when stored or shipped. Samples that cannot be tested immediately should be stored at 2-8 o C for up to 48 hours. For storage beyond 48 hours, freeze specimen -80 o C.

Turnaround Time (TAT):

Reported same day as performed.

Test Availability:

Performed at 9 AM daily (Sun-Sat).

Assay:

2.0 mL serum

Request Form:

Routine Laboratory Request Form

Send To:

UMC MAIN LABORATORY Specimen Receiving – EH319 Extension Hospital

Reference Interval:

Interpretation Range
Negative <0.400 OD
Borderline 0.400 - 0.500 OD
Positive >0.500 OD

Interpretive Data:

Negative (<0.400 OD)

Borderline (0.400 - 0.500 OD) - Repeat in 24-48 hours if clinically indicated

Equivocal (>0.500 OD and <50% inhibition) - Specimen will not be sent to a reference lab for confirmation. Results show reactivity in the presence of heparin, however there is no inhibition of reactivity when excess heparin is added. These results do not support a diagnosis of HIT-related antibody but can be seen in the presence of either HLA or platelet-specific autoantibodies.

Positive (>0.500 OD and >50% inhibition) - Specimen will be sent to a reference lab for confirmatory testing at an additional charge.