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HelpTest Code LAB219 Antistrep-O Titer, Serum
Additional Codes
ASO
Useful For
Demonstration of acute or recent streptococcal infection
Specimen Type
SerumOrdering Guidance
Patients with acute glomerulonephritis following skin infection (post-impetigo) have an attenuated immune response to streptolysin O. For these patients, performance of an alternative streptococcal antibody test such as ADNAS / Anti-DNase B Titer, Serum is recommended.
Specimen Required
Patient Preparation: Fasting preferred but not required
Supplies: Sarstedt 5 mL Aliquot Tube (T914)
Collections Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
On-campus collections: Send in plastic vial, Refrigerated.
Off-campus collections: Centrifuge and aliquot within 1 hour. Specimen to be stored/transported at Refrigerated temp.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days | ||
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | Reject |
| Gross icterus | OK |
Reference Values
<5 years: ≤70 IU/mL
5-17 years: ≤640 IU/mL
≥18 years: ≤530 IU/mL
Interpretation
Elevated values are consistent with an antecedent infection by group A streptococci.
Method Description
In this Siemens Nephelometer II method, the light scattered onto the antigen-antibody complexes is measured. The intensity of the measured scattered light is proportional to the amount of antigen-antibody complexes in the sample under certain conditions. If the antibody volume is kept constant, the signal behaves proportionally to the antigen volume.
A reference curve is generated by a standard with a known antigen content on which the scattered light signals of the samples can be evaluated and calculated as an antigen concentration. Antigen-antibody complexes are formed when a sample containing antigen and the corresponding antiserum are put into a cuvette. A light beam is generated with a light-emitting diode, which is transmitted through the cuvette. The light is scattered onto the immuno-complexes that are present. Antigen and antibody are mixed in the initial measurement, but no complex is formed yet. An antigen-antibody complex is formed in the final measurement.
The result is calculated by subtracting value of the final measurement from the initial measurement. The distribution of intensity of the scattered light depends on the ratio of the particle size of the antigen-antibody complexes to the radiated wavelength.(Instruction manual: Siemens Nephelometer II. Siemens, Inc; Version 2.3, 2008; Addendum to the Instruction Manual 2.3, 08/2017)
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86060
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ASO | Antistrep-O Titer, S | 5370-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ASO | Antistrep-O Titer, S | 5370-2 |
Method Name
Nephelometry